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High expression of MeCP2 in JC virus-infected cells of progressive multifocal leukoencephalopathy brains

机译：MeCP2在进行性多灶性白质脑病脑的JC病毒感染细胞中的高表达

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摘要

Mutations of the methyl CpG binding protein 2 (MeCP2) gene are a major cause of Rett syndrome. To investigate whether the expression of this gene was related to JC virus (JCV) infection, we examined brains of four progressive multifocal leukoencephalopathy (PML) patients. JCV infection was confirmed by immunohistochemical labeling with antibodies against JCV VP1, Agnoprotein and large T antigen. MeCP2 expression was examined by immunohistochemistry using a specific polyclonal antibody against MeCP2. In normal brains and uninfected cortices of PML brains, MeCP2 expression was observed in the nuclei of neurons, but not observed in glial and endothelial cell nuclei. In PML brains, however, intense immunolabeling was observed in abnormally enlarged glial nuclei of JCV-infected cells. Double immunolabeling using antibodies against large T antigen (visualized as blue) and MeCP2 (visualised as red) revealed purple JCV infected nuclei, which confirmed that the JCV infected nuclei expressed MeCP2. We conclude that MeCP2 is highly expressed in the JCV infected nuclei of PML brain and these results may provide a new insight into the mechanism which regulates the MeCP2 expression in glial cells by the infection of JCV.
机译：甲基CpG结合蛋白2（MeCP2）基因的突变是Rett综合征的主要原因。为了调查该基因的表达是否与JC病毒（JCV）感染有关，我们检查了四名进行性多灶性白质脑病（PML）患者的大脑。通过免疫组织化学标记抗JCV VP1，Agnoprotein和大T抗原的抗体，证实了JCV感染。使用针对MeCP2的特异性多克隆抗体，通过免疫组织化学检查了MeCP2的表达。在正常的大脑和未感染的PML大脑皮层中，在神经元核中观察到MeCP2表达，但在神经胶质和内皮细胞核中未观察到MeCP2表达。然而，在PML大脑中，在感染JCV的细胞的神经胶质细胞核中异常放大时观察到了强烈的免疫标记。使用针对大T抗原（显示为蓝色）和MeCP2（显示为红色）的抗体进行的双重免疫标记显示紫色JCV感染的细胞核，这证实了JCV感染的细胞核表达了MeCP2。我们得出结论，MeCP2在受JCV感染的PML脑核中高度表达，这些结果可能为JCV感染调节胶质细胞中MeCP2表达的机制提供新的见解。

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